Fixation buffer.

From:"Dr. Ian Montgomery"

        Preparing fish larvae (sea) for EM, any suggestions for a suitable buffering system given that the levels of salt in sea fish are  ~3 times that of mammals. Do I simply make up the fixatives using sea water? Sorenson buffered fixatives have a high osmolarity that might be another buffer. Time, unfortunately, is short so I can't run a series of trials although I would prefer that.
        Back to mammals, lymphocytes and macrophage in mice, any suggestions. I was going to look for esterases as a marker for macrophage but I'm sure there is something better.

Dr. Ian Montgomery,
Microscopy Service Unit,
Graham Kerr Building,
Institute of Biological & Life Sciences,
University of Glasgow,
G12 8QQ.
Tel: 0141 332 8855 Extn.6644.

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