Fixation buffer.
From: | "Dr. Ian Montgomery" |
Preparing
fish larvae (sea) for EM, any suggestions for a suitable buffering system
given that the levels of salt in sea fish are ~3 times that of
mammals. Do I simply make up the fixatives using sea water? Sorenson
buffered fixatives have a high osmolarity that might be another buffer.
Time, unfortunately, is short so I can't run a series of trials although
I would prefer that.
Back to
mammals, lymphocytes and macrophage in mice, any suggestions. I was going
to look for esterases as a marker for macrophage but I'm sure there is
something better.
Ian.
Dr. Ian Montgomery,
Microscopy Service Unit,
Graham Kerr Building,
Institute of Biological & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 332 8855 Extn.6644.
e-mail: ian.montgomery@bio.gla.ac.uk
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