FW: frozen embryos for LCM
Forgive me if this appears duplicated. I don't think it sent earlier.
> -----Original Message-----
> From: Johnson, Teri
> Sent: Wednesday, December 05, 2001 10:20 AM
> To: Histonet (E-mail)
> Subject: frozen embryos for LCM
> I need some feedback from those of you out there regarding the best
> way to handle/freeze embryos (chick and mouse) for LCM. I looked on
> the NIH site and they suggest immersion in liquid nitrogen after
> wrapping in foil. My question is, how does one mount this on a chuck
> with proper orientation without thawing the specimen and refreezing
> it? Would it be more advisable to use a cryomold to orient the
> specimen in OCT, and then freeze and store it for subsequent cryotomy?
> Also, the site did not indicate what micron thickness to cut the
> frozen sections. Is there a "standard" thickness for frozen section
> as there is for paraffin section? Or is it dependent on the type of
> Thank you for your help, as always.
> Teri Johnson
> Manager Histology Core Facility
> Stowers Institute for Medical Research
> 1000 E. 50th St.
> Kansas City, Missouri 64110
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