Go to

http://members.pgonline.com/~bryand/stain/amyloid/siriusllew.htm

This is a method for amyloid which also demonstrates eosinophils bright red.
Paneth cell granules are also stained.  Depending on the species, elastic
fibres may also be coloured, and I have no idea whether cattle elastic will
stain.

The staining solution has some sodium chloride in it.  This is to overcome
electrostatic repulsion and is almost certainly not needed if the target is
eosinophils, which presumably stain ionically.  Leaving it out will make the
stain darker and the solution will last longer.

Bryan Llewellyn



----- Original Message -----
From: "Rebecca S Smith" <bssvpisu@iastate.edu>
To: "HistoNet Server" <histonet@pathology.swmed.edu>
Sent: December 28, 2000 3:10 PM
Subject: eosinophils


> Does anyone out there in HistoLand know of any selective stains for
> eosinophils, besides Luna's AFIP method with Biebrich Scarlet.  I have a
> scientist wanting something to REALLY selectively stain eosinophils so
that
> Image Analysis can do counts on lots and lots of Bovine tissue slides.  He
> is also staining and counting other types of neutrophils, but is needing
> some Histonet advice on the eosinophil issue.  Thanks in advance!  Happy
> New Millenium!  (the real one this time)
>
>
>