Re: HEP C NS3

From:Luis Chiriboga <luis.chiriboga@med.nyu.edu>

Thanks Andi
I had tried the Tordji-22 when it first came out but also could not get good
consistent results.  I just got the Biodesign clone 861 that marks Core Caspid
protein /MProtein (AA1-124) and  is supposed to work by IHC. I will try it out
next week. We are testing the MMM33 by western as well.  I will fill you in when
we get results. Did you see the 8 December  issue of science?  We are using the
same system for control. Happy Holidays


Kappeler Andreas wrote:

> Hi Luis
> Welcome to the club! In our hands Novocastra's mo-a-HCV, clone MMM33,
> stained about the same proportion of known HCV+ as of control cases ...
> Similar results with clone HCV88 (BioGenex), no positive result with clones
> N57 (HCV NS-3 protein) and C39 (HCV core protein) (both from Biodesign).
> Never tried clone Tordji-22 as a colleague of mine told me - after extensive
> testing - that he had never had any success with this antibody. HCV
> immunohistochemistry still looks like being unsolved. Enjoy the holidays!
>
> Andi Kappeler
> Institute of Pathology, University of Bern, Switzerland

--
Luis Chiriboga Ph.D HT(ASCP)QIHC
Department of Pathology 4W27
Bellevue Hospital
NYU School of Medicine
First Avenue & 27th Street
New York, NY 10016
(212)562-4667





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