Re: CD34 question

From:Michael Rice <>

<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN"> <HTML><HEAD> <META content="text/html; charset=iso-8859-1" http-equiv˙ntent-Type> <META content="MSHTML 5.00.2919.6307" name=GENERATOR></HEAD> <BODY style="FONT: 8pt MS Sans Serif; MARGIN-LEFT: 2px; MARGIN-TOP: 2px"> <DIV><FONT sizeWe are using skin controls and have had no problems with washoff, we do pretty much the same as you however no H2O2, Have you tried different pieces of skin</FONT></DIV> <DIV><FONT sizeMike</FONT></DIV> <DIV><FONT sizeBrow Gen Med Ctr</FONT></DIV> <DIV><FONT sizeFt Laud</FONT><BR><BR>>>> <> 12/28/00 07:22PM >>><BR>Hope that someone can help.  We have just begun using the antibody CD34 in <BR>our lab.  Our pathologists asked us to use skin as a control (even though the <BR>insert specified tonsil)  We are using a Ventana automated stainer, antigen <BR>retrieving in citra buffer by steamer, and then pretreating slides in H2O2. <BR>The patient tissue is staining nicely, but the positive skin control keeps <BR>washing off.  The negative  skin control stays on.  We also ran a positive <BR>tonsil today just to see what it would look like and there was no positive <BR>staining, but at least it stayed on.  Any ideas why the skin would be washing <BR>off ????  AND what does everyone else use for controls?<BR><BR>Thanks for your help<BR>Jennifer Macias, HT (ASCP)<BR>Western Reserve Care System-Forum Health<BR>Youngstown, Ohio<BR><BR></DIV></BODY></HTML>
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