Jerry

I have been using the Sigma MAP-2 antibody (clone HM2) with excellent
results. I carry out microwave retrieval with Citrate buffer and incubate at
1/1000 overnight. Have used on formaldehyde fixed paraffin embedded human
brain tissue (double hemisphere coronal slices) as well as other animal
species. It recognises all 3 MAP-2 isoforms (MAP-2a,2b,2c). If you like I
can send you some details on our publications using the Sigma MAP-2
antibody.

Jim Manavis
Senior Hospital Scientist
Department of Neuropathology
Institute of Medical & Veterinary Science
Adelaide, SA, 5000
Australia
Phone: 61-08-8222-3668
FAX: 61-08-8222 3204
e.mail: jim.manavis@imvs.sa.gov.au
Disclaimer: Not this little black duck!

-----Original Message-----
From:	Jerry Santiago [mailto:SANTIAG@pathology.ufl.edu]
Sent:	Thursday, 28 December 2000 7:13 am
To:	HistoNet@pathology.swmed.edu
Subject:	MAP-2

Is anybody using the antibody MAP-2?  I need some help with this antibody.

Thanks,

Jerry Santiago
Univ. of Florida DRL
Gainesville, FL
352-846-1600