RE: CD34 question

From:"Sebree Linda A." <>

Jennifer, are you by chance using "control" slides from Erie that have the
red squared off area for the positive control and then the area below that
is for the patient tissue?  We've had this same problem where our negative
control stays on fine (on regular "Plus" slides also manufactured by Erie)
but the tissue(s) on the "control" slide will not stay on nearly as well.
We've especially had a problem with bone marrow biopsies.  We've talked to
the folks at Erie and they say there is no difference in the manufacturing
process between the 2 types of slides.

Just a thought to consider. 

Linda A. Sebree, HT
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory, D4/218
600 Highland Ave.
Madison, WI 53792-2472
FAX: (608)263-1568

-----Original Message-----
From: []
Sent: Thursday, December 28, 2000 6:23 PM
Subject: CD34 question

Hope that someone can help.  We have just begun using the antibody CD34 in 
our lab.  Our pathologists asked us to use skin as a control (even though
insert specified tonsil)  We are using a Ventana automated stainer, antigen 
retrieving in citra buffer by steamer, and then pretreating slides in H2O2. 
The patient tissue is staining nicely, but the positive skin control keeps 
washing off.  The negative  skin control stays on.  We also ran a positive 
tonsil today just to see what it would look like and there was no positive 
staining, but at least it stayed on.  Any ideas why the skin would be
off ????  AND what does everyone else use for controls?

Thanks for your help
Jennifer Macias, HT (ASCP)
Western Reserve Care System-Forum Health
Youngstown, Ohio

<< Previous Message | Next Message >>