Microtissue array - new method - the multiblock system
From: | "Dr. Reinhard von Wasielewski" <Wasielewski.Reinhard.von@MH-Hannover.de> |
High to everyone interested in multi-tissue blocks / tissue arrays /
multiblocks !
we do not recommend the Beecher - System. Many people
who have bought this machine (at least I know) have great
difficulties with it. Waste of time and loss of tissue due to lack of
adhesion is a very common problem. Zytomed (www.zytomed.de) offers a
multiblock system which allows to bring up to 96 tissues in one
multiblock. The system is different from the beecher system, i.e. you
punsh your tissue cores of the interested area of your archival
block by yourself (1.5 mm in diameter) and send them to zytomed. All
you need in included in the kit you have to buy from zytomed (partner
of zymed). The multiblock you receive can be cut like any standard
paraffin block, you need no special equipement. Depending on the
thickness of the tissue cores brought into the multiblock, up to 150
slides can be achieved on a routine microtome. Therefore, the
technique is especially interesting for studying several markers on
the same tissues. No tape system !
We have used this multiblock system in a nationwide immuno-trial this
spring in Germany. We sent out 6 slides of a multiblock containing 30
tissue cores to 172 laboratories (6x30x172 =approx. 31.000 cores).
They were sent back to us after staining for ER, MIB1, Keratin,
...(i.e. including heat retrieval methods). Less than 0.4% of the
31000 cores were lost due to adhesion problems (in 172 different
laboratories !!!), which is a very low number that corresponds well
to loss on standard slides (or even below). The multiblock system is
also appropriate for In-Situ-hybridization, we have investigated more
than 2000 cases up to now in 34 multiblocks with no real problems.
For starting with the multiblock system, we recommend blocks with 60
tissue cores (plus one core for orientation). This reduces the
cutting area and makes the whole process for you and your staff
easier. But the greatest difference is the storeability of the
punshed tissue cores ! Once you are punshing your archival blocks-
just do it ten times per paraffin block and you can build up your own
tissue-core-archive ! Think of all the possibilities you will have in
future studies: for example, 420 tumors are brought into 7
multiblocks. After screening for certain markers, your results may
recommend investigating just 120 of these tumors in detail - now you
can construct two new multiblocks very fast and easy including only
the cases of special interest by using your tissue-core-archive ! And
if you are interested in the DNA, just extract it from one of the
stored tissue cores after you have screened the cases for the most
interesting ones !
For further information just contact me (or www.zytomed.de)
With best regards
Reinhard von Wasielewski, Hannover, Germany
Reinhard von Wasielewski, MD
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