Dear Anila,

The reason you use frozen tissue instead of wax embedding depends on the
antigen I want to see.

Some antibodies will only work on frozen material and others only on
embedded material.

I am now working alot more with embedded tissue, but I have to admit that I
do perfer working with frozen material. I think the staining for IHC is alot
cleaner. As long a the material is frozen properly the morphology can be
alomst as good as that for embedded tissue.

Tammaryn Lashley
Dep. of Neuropathology
Institute of Neurology
Queens Square House
Queens Square
London WC1N 3BG

Tel: 0171 837 3611 ext 4247