Vibratome sectioning advice
|From:||"Richard L. Young" <firstname.lastname@example.org>|
A further point to ponder.
I am keen to cut some small ganglia on a vibrotome (40-50 micron)
in preparation for confocal fluorescent microscopy and need to
maximise my section count. I there a semi-rigid support or
embedding media that would allow me to mount these flat, soft
ganglia to successfully section in transverse rather than
I am considering a concentrated agar solution in which to embed,
but conscious of needing to retain the ability to secure the base of
any block to the chuck using supa-glue.
Any takers ? Thanks in advice.
Richard L. Young, PhD
Nerve-Gut Research Laboratory
Royal Adelaide Hospital
Level 1, Hanson Centre
Adelaide, South Australia 5000
Phone: +61 8 8222 2077
Fax: +61 8 8222 5934
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