Vibratome sectioning advice

From:"Richard L. Young" <>

Dear Histonetters, 

A further point to ponder. 
I am keen to cut some small ganglia on a vibrotome (40-50 micron) 
in preparation for confocal fluorescent microscopy and need to 
maximise my section count. I there a semi-rigid support or 
embedding media that would allow me to mount these flat, soft 
ganglia to successfully section in transverse rather than 
longitudinal ?

I am considering a concentrated agar solution in which to embed, 
but conscious of needing to retain the ability to secure the base of 
any block to the chuck using supa-glue.

Any takers ? Thanks in advice.


Richard L. Young, PhD

Nerve-Gut Research Laboratory
Royal Adelaide Hospital
Level 1, Hanson Centre
Frome Road
Adelaide, South Australia 5000
Phone: +61 8 8222 2077
Fax: +61 8 8222 5934

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