TEM on fecal samples, vet diagnostic protocol

From:Gayle Callis <uvsgc@msu.oscs.montana.edu> (by way of Histonet)

Our EM lab does bovine fecal samples for veterinary diagnositic TEM, fixed
and with a special protocol.   If interested, contact, contact our EM
Technician, Andy Blixt at uvsjb@montana.edu.  He does not participate on
Histonet, it would be personal communication.

Could save you a lot of extra work cleaning potential contamination from
biohazardous material.

At 03:45 PM 12/12/00 -0600, you wrote:
>Colleagues, a pathologist asked to use our Transmission Electron
>Microscope(TEM) and when I was going to help him load his copper grids, he
>said he would do it as it was a fecal preparation for viral studies.  I
>found out that material on formar coated copper grids was not fixed. After
>he viewed (no photos taken) I pulled the copper grid holder and all items
>touched and ultrasonicated with absolute ethanol and then acetone and then
>placed items without touching in a 70oC oven for an hour.  I feel that is
>enough, but would like to know if anyone else has been in this predicament.
>Thanks, Teresa
Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303

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