Re: mouse mammary tumor Ab
|From:||Jan Shivers <firstname.lastname@example.org> (by way of Histonet)|
Chris (and any one else who might have some information),
I guess I should have been more clear in my request for mouse mammary tumor
Abs. Yes, I assume that human cytokeratin Abs will cross-react with the
mouse tissue. They have done so on all other species that I've used them on
here in the Vet Diag Lab. What I need is an antibody specific for mammary
tumor, so that we can differentiate these tumors from salivary gland tumors,
etc. I need to be able to say that it is more than just an epithelial
tumor, or even more than a glandular or ductual epithelial tumor. Is there
such a beast that will work in mouse tissue?
And thanks for the recommendation of using the ARK kit from DAKO. I do have
one on hand.
----- Original Message -----
From: "C.M. vander Loos" <email@example.com>
Cc: "Jan Shivers" <firstname.lastname@example.org>
Sent: Thursday, December 14, 2000 2:58 AM
Subject: Re: mouse mammary tumor Ab
> >Does anyone know where I could find an antibody that detects mouse
> >tumor cells (or human mammary tumor cells, but cross-reacts with mouse
> >Also, the doctor who collected the mouse tissue has had it sitting in
> >alcohol for who knows how long. Will this pose a problem with antigen
> >detection? I have only worked with formaldehyde-fixed or frozen tissues.
> >Jan Shivers
> >Univ. of Minnesota
> >Veterinary Diagnostic Lab - IHC
> >1333 Gortner Ave.
> >St. Paul, MN 55108
> >612-625-1732; 612-624-7297
> Since a number of anti-human cytokeratin antibodies tend to crossreact
> epithelia from other species, there is a fair chance that e.g.
> anti-cytokeratin MNF116 (DAKO) will also crossreact with mouse tissue.
> Solving the problem of mouse antibodies on mouse tissue, the Animal
> Research Kit (DAKO) can be a great tool.
> I don't think that alcohol-fixation will be a problem, if you take some
> tissue thrinking for granted. Consider a 24-48 hours extra
> fixation/emersion in Methacarn (60% methanol, 10% acetic acid-glacial, 30%
> chloroform). However, be sure that upon tissue processing the samples does
> not go into formalin. Alcohol-fixed tissue sections do not need any
> pretreatment with heat-induced antigen retrieval or proteolytic enzymes
> because the fixation type is non-crosslinking.
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