Re: decalcification process

From:Lesley Weston <lesley@interchange.ubc.ca>

If all else fails, you could try clearing in cedarwood oil (See Culling
for method). This takes a long time, but it leaves the decalcified tissue
much more cuttable than xylene or even chloroform.

Lesley Weston.



On Thu, 7 Dec 2000, Neuropathology wrote:

> Keep in mind that you may have completed decacification.  Your cutting
> problems might be reduced by treating with something like mollifex or fabric
> conditioner.
> Andy Shand
> ----- Original Message -----
> From: Mack, Jacquie <Jacquie.Mack@CLS.ab.ca>
> To: Histonet information (E-mail) <Histonet@pathology.swmed.edu>
> Sent: Wednesday, December 06, 2000 4:50 PM
> Subject: decalcification process
> 
> 
> >
> > Does anyone have any suggestions regarding decalcifying cortical bone?  We
> > have a mandible here we've been trying to decalcify for weeks.  17% formic
> > acid, then RDO (a commercial rapid decalcified).  We are still
> encountering
> > a lot of problems cutting sections on our blocks!
> >
> > JACQUIE MACK
> > TECH II MAIN LAB FMC
> > HISTOPATHOLOGY
> > PH: 670-1574
> > e mail:  Jacquie.Mack@CLS.ab.ca
> >
> >
> 
> 
> 




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