RE: in situ question

From:"Connolly, Brett M" <>


There are X and Y cDNA probes around that are used in chromosome painting
aka skypainting. I am not sure if they can be used in other ISH
applications, though. Roche (formerly Boerhinger Mannheim) has them as does
Spectral Imaging
( Try calling
their tech services for help. I would think a Medline search wopuld pull up
something useful, too.


Brett M. Connolly, Ph.D.
Merck Research Laboratories
Department of Pharmacology
PO Box 4
West Point, PA 19486
Ph. 215-652-2501
FAX 215-652-2075

> ----------
> From: 	Denise Bland-Piontek[]
> Sent: 	Tuesday, December 19, 2000 2:01 PM
> To:
> Subject: 	in situ question
> Does anyone know of a DNA specific probe for either the x or y
> chromosome? I've checked several suppliers of in situ probes to no
> avail, but no one seems to know if such probes exist or not? I've been
> asked by a researcher if either of these probes exist and will they work
> on paraffin embedded human tissue? Any advice is appreciated!
> Denise Bland-Piontek, HTL (ASCP)
> Scientist
> Anatomic Pathology Research Lab
> University of Minnesota

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