Denise,

There are X and Y cDNA probes around that are used in chromosome painting
aka skypainting. I am not sure if they can be used in other ISH
applications, though. Roche (formerly Boerhinger Mannheim) has them as does
Spectral Imaging
(http://www.spectral-imaging.com/website/webpages/probes.html). Try calling
their tech services for help. I would think a Medline search wopuld pull up
something useful, too.

Regards,
Brett


Brett M. Connolly, Ph.D.
Merck Research Laboratories
Department of Pharmacology
WP26A-3000
PO Box 4
West Point, PA 19486
Ph. 215-652-2501
FAX 215-652-2075
e-mail: brett_connolly@merck.com
 

> ----------
> From: 	Denise Bland-Piontek[SMTP:bland008@umn.edu]
> Sent: 	Tuesday, December 19, 2000 2:01 PM
> To: 	histonet@pathology.swmed.edu
> Subject: 	in situ question
> 
> Does anyone know of a DNA specific probe for either the x or y
> chromosome? I've checked several suppliers of in situ probes to no
> avail, but no one seems to know if such probes exist or not? I've been
> asked by a researcher if either of these probes exist and will they work
> on paraffin embedded human tissue? Any advice is appreciated!
> Denise Bland-Piontek, HTL (ASCP)
> Scientist
> Anatomic Pathology Research Lab
> University of Minnesota
> 
> 
>