I did some ISH studies with mouse bones and found that they could not be
decalcified, we ended up cutting frozen sections without decal using the
Instrumedics tape transfer sectioning system.
Patsy 

		-----Original Message-----
		From:	Patrick M. Haley  [mailto:pmhales@cybergap.net]
		Sent:	Wednesday, December 06, 2000 6:39 PM
		To:	Histonet
		Subject:	In-situ microtomy

		Fellow Netters,
		    I have  a researcher wanting to perform in-situ on mouse
feet. They are
		looking at an arthritis model. The specimens will be fixed
in
		Methyl-Carnoy's. I have two questions:

		Can these be decalcified without loosing DNA/RNA, and what
is the reagent of
		choice?
		If they can not be decalcified, what is the best method of
microtomy  to
		produce usable sections?

		Many Thanks!!!

		Pat Haley
		HTN, inc.
		www.histology.net