RE: In-situ microtomy

I did some ISH studies with mouse bones and found that they could not be
decalcified, we ended up cutting frozen sections without decal using the
Instrumedics tape transfer sectioning system.

		-----Original Message-----
		From:	Patrick M. Haley  []
		Sent:	Wednesday, December 06, 2000 6:39 PM
		To:	Histonet
		Subject:	In-situ microtomy

		Fellow Netters,
		    I have  a researcher wanting to perform in-situ on mouse
feet. They are
		looking at an arthritis model. The specimens will be fixed
		Methyl-Carnoy's. I have two questions:

		Can these be decalcified without loosing DNA/RNA, and what
is the reagent of
		If they can not be decalcified, what is the best method of
microtomy  to
		produce usable sections?

		Many Thanks!!!

		Pat Haley
		HTN, inc.

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