RE: In-situ microtomy
I did some ISH studies with mouse bones and found that they could not be
decalcified, we ended up cutting frozen sections without decal using the
Instrumedics tape transfer sectioning system.
From: Patrick M. Haley [mailto:firstname.lastname@example.org]
Sent: Wednesday, December 06, 2000 6:39 PM
Subject: In-situ microtomy
I have a researcher wanting to perform in-situ on mouse
feet. They are
looking at an arthritis model. The specimens will be fixed
Methyl-Carnoy's. I have two questions:
Can these be decalcified without loosing DNA/RNA, and what
is the reagent of
If they can not be decalcified, what is the best method of
produce usable sections?
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