I have done thousands  of gms always use fresh chromic each time for one
hour...have never heated it...This help to avoid wash off also...plus u go
to lunch during the time...(:-)

-----Original Message-----
From: Kimcatk@aol.com [mailto:Kimcatk@aol.com]
Sent: Thursday, December 07, 2000 12:47 PM
To: histonet@pathology.swmed.edu
Subject: GMS & Steiner Chapman / Re: Seeking Staining Procedures


Dear HistoNet colleagues,
     It is great to have HistoNet working again!  Thank you to those of you
who responded to my posting.  I got some leads on staining for fungi and
spirochetes, but I still have some questions.  
     Has anyone done Grocott's Methenamine Silver (GMS) without heating the
chromic acid?  I heard that it is possible to oxidize in the chromic acid
for an hour instead of heating it, but I do not have a procedure.
     Also, I am looking for a procedure for Steiner Chapman because that
replaces the uranium/uranyl nitrate with some type of zinc solution.
     I would be very grateful for any help you could provide.

Sincerely,

Kimberly Atkin HT (ASCP)
Boston, MA


In a message dated Tue, 5 Dec 2000  8:52:00 AM Eastern Standard Time,
Kimcatk writes:

<< Dear HistoNet colleagues,

     I am seeking to replace some staining procedures in my laboratory with
NON-microwave methods.  Optimally, I am hoping to find stains that are free
of heavy metals or any particularly toxic or dangerous components.  I can
not use mercury.

     I need to find staining protocols or kits to demonstrate the following:

Fungal organisms
Spirochetes, Campylobacter, and Legionella organisms

     In addition, the gram stain procedure I use is very cumbersome and I
would be extremely grateful for a simpler method.
     
     Thank you all very much in advance for your help.

Sincerely,

Kimberly Atkin HT (ASCP)

Boston, Massachusetts

 >>