I keep EDTA refrigerated and use it for months.  In my experience shaking
(on a platform shaker) or oscillating somehow during the decal process is
very important and speeds the process.  I also wash in running di water
after acid and edta decalcification.  I have done comparative studies with
EDTA and 5% formic acid decal for IHC and found that the formic acid is just
as good for my purposes and faster.
Patsy Ruegg

-----Original Message-----
From: HOOVER_JENNIFER@LILLY.COM [mailto:HOOVER_JENNIFER@LILLY.COM]
Sent: Monday, December 18, 2000 11:28 AM
To: HistoNet@pathology.swmed.edu
Subject: EDTA as Decalcifying Agent?



        Hello Histonetters, 

                I have  a few questions regarding EDTA as a decalcifying
agent.  1)  why is it not more commercialized (ready-made) which leads to
question  2)  when I prepare EDTA in the laboratory what is the approximate
shelf life?  I have read my histology books and found reference to EDTA but
I know the veteran histonetters and bone researchers are probably proficient
with knowledge on this particular subject.  Also, are there any good
reference articles out there dealing with decalcifying agents and effects on
tissue?  I also need some feedback on how important is it to rinse the
tissue in running tap water after acid decalcification to remove acid
residue before processing?  This has been a topic of debate.  As always I am
forever grateful for any knowledge, wisdom or experience from Histoland.
Thank you! 



Jennifer Hoover 
Eli Lilly and Company