Fw: manual vs automated IHC

From:Jan Shivers <shive003@maroon.tc.umn.edu>


I wholeheartedly agree with Patsy in the advantage of using a DAKO stainer.
In my lab it is absolutely necessary for me to use my own antibody
dilutions, since I work on several species at a time.  An open system allows
me to do just that.  Besides, with the DAKO stainer, you can actually SEE
the slides as they are being stained.  Being from the old school, I like to
see what's going on at all times.  My daily workload is usually 96

DAKO technical and maintenance services are excellent.

Jan Shivers
U of MN Vet Diag Lab

-----Original Message-----
From: Patsy.Ruegg@uchsc.edu <Patsy.Ruegg@uchsc.edu>
To: tomers@home.com <tomers@home.com>; histonet@pathology.swmed.edu
Date: Saturday, December 02, 2000 10:46 AM
Subject: RE: manual vs automated IHC

>i think you would benefit from the use of a DAKO autostainer.  it does up
>48 slides at one time and is an open system so that you can use any
>detection you want. some of the other autostainers require that you use
>their prepackaged detection systems and can be very expensive to use.
>Patsy Ruegg
>-----Original Message-----
>From: Tom Wells
>To: 'histonet'
>Sent: 12/1/00 9:58 AM
>Subject: manual vs automated IHC
>We are in the process of automating our IHC lab. I am curious as to the
>number of labs who are automated vs. the ones who still use manual
>We average between 20 to 30 slides per day. Any feedback would be
>appreciated. Thanks. Tom.
>Tom Wells, BSc, ART
>Supervisor, Immunohistochemistry Laboratory
>Lions Gate Hospital
>North Vancouver, BC

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