Fab2-FITC secondaries
| From: | John Bliss <bliss@zymed.com> |
--------------9854C0D0FDA568E0E97490EE
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
Dear Dennis:
Zymed offers Goat F(ab')2 anti-Rabbit IgG (H+L)-FITC, cat. no. 62-1811.
Sincerely,
John Bliss
--
>Dear All:
>
>I was quite pleased with my batch of Fab2-FITC (goat
>anti-rabbit) secondary from Boehringer
>Mannheim. Now I'm out. Roche bought up B/M (in the spirit of leaner
and
>meaner management style) and Roche no longer sells the antibody. The
>Roche tech rep recommended Chemicon. The best I could get shines real
>green but sticks to the
>mouse tissue I'm working on these days (Head and parts thereof). I use
>plenty of normal serum and 5% BSA so I don't think the blocker is the
>problem. I think the antibody is the problem.
>
>Does anyone out there have a favorite anti-rabbit IgG to recommend?
>
>
>
>John Carroll Dennis
>Anatomy, Physiology, and Pharmacology
>109 Greene Hall
>Auburn University, AL 36849
--------------9854C0D0FDA568E0E97490EE
Content-Type: text/html; charset=us-ascii
Content-Transfer-Encoding: 7bit
<!doctype html public "-//w3c//dtd html 4.0 transitional//en">
<html>
Dear Dennis:
<p>Zymed offers Goat F(ab')2 anti-Rabbit IgG (H+L)-FITC, cat. no. 62-1811.
<p>Sincerely,
<p>John Bliss
<p>--
<p>><i>Dear All:</i>
<br><i>></i>
<br><i>>I was quite pleased with my batch of Fab2-FITC (goat</i>
<br><i>>anti-rabbit) secondary from Boehringer</i>
<br><i>>Mannheim. Now I'm out. Roche bought up B/M (in the
spirit of leaner and</i>
<br><i>>meaner management style) and Roche no longer sells the antibody.
The</i>
<br><i>>Roche tech rep recommended Chemicon. The best I could get
shines real</i>
<br><i>>green but sticks to the</i>
<br><i>>mouse tissue I'm working on these days (Head and parts thereof).
I use</i>
<br><i>>plenty of normal serum and 5% BSA so I don't think the blocker
is the</i>
<br><i>>problem. I think the antibody is the problem.</i>
<br><i>></i>
<br><i>>Does anyone out there have a favorite anti-rabbit IgG to recommend?</i>
<br><i>></i>
<br><i>></i>
<br><i>></i>
<br><i>>John Carroll Dennis</i>
<br><i>>Anatomy, Physiology, and Pharmacology</i>
<br><i>>109 Greene Hall</i>
<br><i>>Auburn University, AL 36849</i></html>
--------------9854C0D0FDA568E0E97490EE--
<< Previous Message | Next Message >>