<br><font size=2 face="sans-serif"> Hello Histonetters,</font>
<br>
<br><font size=2 face="sans-serif"> I have a few questions regarding EDTA as a decalcifying agent. 1) why is it not more commercialized (ready-made) which leads to question 2) when I prepare EDTA in the laboratory what is the approximate shelf life? I have read my histology books and found reference to EDTA but I know the veteran histonetters and bone researchers are probably proficient with knowledge on this particular subject. Also, are there any good reference articles out there dealing with decalcifying agents and effects on tissue? I also need some feedback on how important is it to rinse the tissue in running tap water after acid decalcification to remove acid residue before processing? This has been a topic of debate. As always I am forever grateful for any knowledge, wisdom or experience from Histoland. Thank you!</font>
<br>
<br>
<br>
<br><font size=2 face="sans-serif">Jennifer Hoover</font>
<br><font size=2 face="sans-serif">Eli Lilly and Company</font>