eosin/thymol

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From:Gayle Callis <uvsgc@msu.oscs.montana.edu>
To:histonet@pathology.swmed.edu
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Date:Sun, 29 Aug 1999 20:07:06 -0600
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I find the concept of adding thymol to eosin interesting.  If the alcohol content is
70%, I doubt you will ever get growth, as this is the concentration preferred by those
working in biohoods, to kill off bacterial and probably other contaminants and that is 
why they spray and wipe down all working surfaces, before and after handling specimens or
cell cultures.

If you are working with aqueous solutions of eosin, you may have a problem, have seen
this in stock1% eosin, before making up eosin phloxine solutions, even in the phloxine,
but I can honestly say, I have never seen growth in any commercial preparations or 
alchololic eosin solutions.  If you are not making up huge stocks, and use them fairly soon,
plus storage in refrig, you may not need to use the thymol.  Vendors, what do you do?
Your silence is overwhelming!

Gayle Callis   



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