Resistance to experimenting (was: times given in procedures)

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From:"Tim Morken" <timcdc@hotmail.com>
To:histonet@pathology.swmed.edu
Reply-To:
Date:Tue, 31 Aug 1999 07:41:20 EDT
Content-Type:text/plain; format=flowed

Steven Slap wrote

>Individual labs need to understand that they may have to experiment to 
>optimize procedures, but there is a growing resistance to this idea.<

This is an interesting problem. What sort of people are coming to work in 
the lab who are not willing to experiment to get the best results? I wonder 
if pre-packaged reagent kits and automation are working against us us this 
regard.

Tim Morken, B.A., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9@cdc.gov
       timcdc@hotmail.com

Phone: (404) 639-3964
FAX:  (404)639-3043










----Original Message Follows----
From: "Slap, Steven" <SSlap@ebsciences.com>
To: Barry Rittman <brittman@mail.db.uth.tmc.edu>,        histology 
<histonet@pathology.swmed.edu>
Subject: times given in procedures
Date: Mon, 30 Aug 1999 10:44:28 -0400

Dear Histonetters,

Barry Rittman wrote:
	As noted by several others here, the times for most techniques
are guides only
	to provide a range. The results depend on many  factors such as
different
	fixation and processing techniques and times, thickness of
section, type of
	tissue and final result that is desired.

I couldn't agree more.  As a vendor of microwave processors, I am always
being asked for fixation and processing schedules which are ideal for
all specimens under all possible conditions.  Individual labs need to
understand that they may have to experiment to optimize procedures, but
there is a growing resistance to this idea.  In my workshops, I try to
explain the principles behind the process so that trained technologists
will understand the impacts of varying certain parameters.

Best regards,
Steven Slap

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