Re: staining times

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From:Ken Turner <>
To:"Ronnie Houston" <>
Date:Fri, 27 Aug 1999 09:24:57 +1200
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I couldn't agree with you more. I have found the same level of adolation of
'timers' by students (and staff alike) often to the detriment of the final
appearance of the stain in use. I suppose those less experienced are
reluctant to trust their own observations and tend to rely too heavily on
the 'timer'. Like yourself, my first recourse is to a macroscopic
observation followed, if necessary, by a microscopic observation. Of
course, there are some techniques which require critical timing, these can
always be highlighted on running protocols, and, as you intimate, times may
vary from lab to lab or from tissue to tissue. 


At 09:05 AM 8/26/99 CDT, you wrote:
>A recent question about the staining time of hematoxylin alludes to the 
>point of the recommended time in staining protocols.
>This time is just that......that recommended by the author(s), but does not 
>reflect what is required for your own conditions. The microscope is the 
>ultimate control, and not what is written down in a cookbook-type method.
>I have found that newer students to the profession tend to place too much 
>emphasis on exact staining times and not on microscopic control.
>Just my observation, but would appreciate comments.
>Ronnie Houston
>Cytochemistry & Molecular Pathology
>Texas Scottish rite Hospital for Children
>Get Free Email and Do More On The Web. Visit
Kenneth W Turner
Senior Teaching Fellow
Manager Histology Service Unit
Department of Pathology			
Dunedin School of Medicine		e-mail	
University of Otago			Phone:  (03) 479 7135 or (03) 479-7152
New Zealand				Fax:    (03) 479-7136

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