Re: re-staining faded slides

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From:"Andrea Kelly" <canislupus@albanyonline.net>
To:"Carla Aiwohi" <Carla_Aiwohi@usgs.gov>, <HistoNet@pathology.swmed.edu>
Reply-To:
Date:Fri, 20 Aug 1999 21:41:27 -0400
Content-Type:text/plain; charset="iso-8859-1"

I would take them back as you planned only go all the way to water.Then go
to 0.5-1% acid alcohol to destain (about 1-3 minutes). Wash in water then
rinse in distilled.Have you seen Charles Churukian's Staining Manual?
Besides all of the wonderful microwave methods there is a ton of useful
information such as destaining, shelf life of solutions/stains, how to get
specific stains out of clothing, hands and glassware. It is very reasonable
too.

Andrea Kelly
-----Original Message-----
From: Carla Aiwohi <Carla_Aiwohi@usgs.gov>
To: HistoNet@pathology.swmed.edu <HistoNet@pathology.swmed.edu>
Date: Friday, August 20, 1999 1:05 PM
Subject: re-staining faded slides


>Hi everyone,
>
>    I have three faded slides that were stained with PAS, H&E and
Richardson's
>about 10 years ago.  Can anyone offer advice as to the best way to
rejuvenate
>them?  I had planned to soak them in xylene, take slides to 70% then
re-stain
>using our usual procedures.  Thanks for your help and have a good weekend.
>
>Carla Aiwohi
>Western Fisheries Research Center
>Seattle, WA
>
>




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