Hi Gayle,
I have done the Dawson's technique published in CFA Culling's third edition
of "Handbook of HIstopathological and Histochemical Techniques" on page 549,
but only uses Alizarin red S.  It works very nicely and I still have the
specimen that I used it on, still preserved.

Shirley Powell

Gayle Callis wrote:

> I have seen fetuses stained with alizarin red S and alcian blue.
>
> Cartilage vs bone is clearly distinguished, and
> the samples where stained as whole mounts. Have never done it, but seen
> photos in publications, spectacular preparations!  Hopefully someone who
> has done this, will come forth with their tried and true method.
>
> Gayle Callis