Re: ossification
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From: | "Barry Rittman" <brittman@mail.db.uth.tmc.edu> |
To: | histonet@pathology.swmed.edu |
Reply-To: | |
Date: | Thu, 26 Aug 1999 19:14:45 -0700 |
Content-Type: | text/plain; charset="us-ascii" |
Gayle,
this is the Spalteholz technic and a series of modifications. Its only
problem is one of time. I thought that the original question was asking
about distinguishing bone and cartilage on processed sections. If for whole
mounts then I can provide some techniques.
Barry
At 10:07 AM 08/26/1999 -0600, you wrote:
>I have seen fetuses stained with alizarin red S and alcian blue.
>
>Cartilage vs bone is clearly distinguished, and
>the samples where stained as whole mounts. Have never done it, but seen
>photos in publications, spectacular preparations! Hopefully someone who
>has done this, will come forth with their tried and true method.
>
>Gayle Callis
>
>
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