Re: need murine kidney specialist

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From:"Tony Henwood" <>, Marcia Bentz <>
Date:Wed, 25 Aug 1999 20:50:11 +0000
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Dear Marcia,

> Just me again pulling my hair out with the cytokine staining on mouse
> kidney. Still getting inappropriate signal on negative control (frozen
> sections), but it seems to be specific for tubular epithelial cells. Is
> there anything special about these cells that would make them "sticky" with
> an ABC peroxidase kit? Interestingly, the kidney treated with LPS seemed to
> show significantly less of this than the untreated kidney.

The tubular cells are rich in biotin so they will bind to the avidin 
(or streptavidin). Try the old PAP technique of Sternberger, this 
might help.
Regards, Tony.
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital

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