Re: humidity chamber

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From:"Hiroaki Nitta" <>
To:"Rebecca Hartley" <>, "HistoNet Server" <>
Date:Mon, 16 Aug 1999 23:21:43 -0400
Content-Type:text/plain; charset="iso-8859-1"

I used to do it like you mentioned in  your post when I was a graduate
student at University of Illinois years ago.  (Oh, I am getting old.)   But,
since I used SlideMaster for both IHC and ISH, I just can't imagine doing
IHC and ISH without using SlideMaster.  Unfortunately, the original
SlideMaster is no longer had some problems, but I loved the
system so much.

Hiro Nitta
-----Original Message-----
From: Rebecca Hartley <>
To: HistoNet Server <>
Date: Friday, August 13, 1999 4:29 PM
Subject: : humidity chamber

>Hi all -
>Yup - I make my own humidified chambers.  I currently use Tupperware boxes
>with 10 ml plastic pipettes taped to the bottom of the Tupperware (to lay
>the slides on) and a wet Kim wipe in between the pipettes.  The pipettes
>be cut to whatever length is best for each Tupperware size.  Works peachy
>and is incredibly versatile - you can move the pipettes into any separation
>distance or whatever, the covers fit nice and snug, etc.
>Rebecca Hartley, Ph.D.
>Layton BioScience, Inc.
>Gilroy, CA
>Date: 12 Aug 1999 10:40:33 -0500
>From: Karen S Pawlowski <>
>Subject: Re: humidity chamber
>Hi Brett,
>A long time ago a person in the lab I worked in did large groups of slides
>in a 9 X 13 cake pan with lid.  Just put some wet paper towels at the
>bottom and let the slides sit on a pair of applicator sticks....  Anyone
>still use this method?
>Karen Pawlowski
>Sr. Res. Assoc., UT SW Med. Ctr./
>PhD Candidate, UT Dallas
>Dallas, TX

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