Rebecca:
I used to do it like you mentioned in  your post when I was a graduate
student at University of Illinois years ago.  (Oh, I am getting old.)   But,
since I used SlideMaster for both IHC and ISH, I just can't imagine doing
IHC and ISH without using SlideMaster.  Unfortunately, the original
SlideMaster is no longer available....it had some problems, but I loved the
system so much.

Hiro Nitta
-----Original Message-----
From: Rebecca Hartley <rebeccahartley@earthlink.net>
To: HistoNet Server <HistoNet@pathology.swmed.edu>
Date: Friday, August 13, 1999 4:29 PM
Subject: : humidity chamber


>Hi all -
>
>Yup - I make my own humidified chambers.  I currently use Tupperware boxes
>with 10 ml plastic pipettes taped to the bottom of the Tupperware (to lay
>the slides on) and a wet Kim wipe in between the pipettes.  The pipettes
can
>be cut to whatever length is best for each Tupperware size.  Works peachy
>and is incredibly versatile - you can move the pipettes into any separation
>distance or whatever, the covers fit nice and snug, etc.
>
>Rebecca
>
>Rebecca Hartley, Ph.D.
>Layton BioScience, Inc.
>Gilroy, CA
>
>
>Date: 12 Aug 1999 10:40:33 -0500
>From: Karen S Pawlowski <kna101@utdallas.edu>
>Subject: Re: humidity chamber
>
>Hi Brett,
>
>A long time ago a person in the lab I worked in did large groups of slides
>in a 9 X 13 cake pan with lid.  Just put some wet paper towels at the
>bottom and let the slides sit on a pair of applicator sticks....  Anyone
>still use this method?
>
>Karen
>
>Karen Pawlowski
>Sr. Res. Assoc., UT SW Med. Ctr./
>PhD Candidate, UT Dallas
>Dallas, TX
>
>
>