depends on what you want and what you are counterstaining but basically most
hematoxylins will work.
have to remember that picric acid in Bouin's fixative is a coarse precipitant
of protein so that the nuclear image you see may be a lot different to that
using other fixatives.
Barry

deshanie ganessunker wrote:

> Histonetters,
> I will be using hematoxylin as  a counterstain for bouin's-fixed
> intestinal tissue. Does it matter what kind of hematoxylin I should use
> and whether I need to use it full-strength or diluted. I am a grad student
> in Nutritional Sciences and totally ignorant about this area and I greatly
> appreciate your response.
> Thanks in advance
> Deshanie Ganessunker
> U of I-Urbana