Re: Trichrome stains on kidneys

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From:denise M m Long-Woodward <>
Date:Wed, 18 Aug 1999 02:30:01 +0000

I don't know if this will help given the fixative issue but I recently
had to do Trichromes on mouse skin. The Gomori's solution purchased was
also giving inaccurate colors.  The vendor informed me the optimal pH for
a Gomori's Trichrome solution is pH 1.3. At that pH, the muscle fibers
selectively take up the red. After adjusting the pH to 1.3 with HCl, it
worked beautifully.   
denise woodward

On Mon, 16 Aug 1999 16:20:58 -0500 "Jennings-Siena, Debbie"
<> writes:
> Hi is anyone else out there using a Gomori's Trichrome on kidney's?  
> We are
> having some trouble with our results, the pathologist says that the 
> colors
> are not right.  If  your lab has some wonderful new procedure or 
> even a
> wonderful old procedure that works great, could you let me know.  
> Our kidney
> biopsies are fixed in Helley's fixative and that may be our problem.
> Anyway, Thanks for your help.
> Debbie Jennings-Siena
> Baylor University Medical Center
> Dallas, Texas 75246
> 214-820-2465

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