Re: Trichrome stains on kidneys

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From:"Sarah Christo" <>
To:<>, <>
Date:Tue, 17 Aug 1999 08:02:54 -0500
Content-Type:text/plain; charset=US-ASCII

Dear Debbie,
   The procedure I use works every time.  It is basically the Masson's Trichrome from the AFIP manual.  The one trick I learned from a tech with 25 years experience is after the Biebrich Scarlet-Acid Fuchsin to rinse in distilled water  until the red is bled out.   Since I'm usually staining hoof lamina I just let the slides sit in DI (not washing under a flow of water) until I don't see the red coming off the sections anymore.  I'll change the water a few times during the process.  I do all the reagent steps for 5 minutes.  Hope this helps.  Good luck,  Sarah

Sarah Christo, HT (ASCP)
Texas A&M University
College of Veterinary Medicine
Dept. of Vet. Anatomy & Public Health
College Station, TX  77868-4458

>>> "Jennings-Siena, Debbie" <> 08/16 4:20 PM >>>
Hi is anyone else out there using a Gomori's Trichrome on kidney's?  We are
having some trouble with our results, the pathologist says that the colors
are not right.  If  your lab has some wonderful new procedure or even a
wonderful old procedure that works great, could you let me know.  Our kidney
biopsies are fixed in Helley's fixative and that may be our problem.
Anyway, Thanks for your help.
Debbie Jennings-Siena
Baylor University Medical Center
Dallas, Texas 75246

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