Re: H&E on 50um brains

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From:"Sarah Christo" <>
To:<>, <>
Date:Thu, 19 Aug 1999 08:27:11 -0500
Content-Type:text/plain; charset=US-ASCII

Dear Emily,
   I use Mayer's Hematoxylin for 10 minutes on 50 micron sections.  Differentiation is the problem with the thick sections.  I use my routine 1% al. eosin for 2 minutes.   

Sarah Christo, HT (ASCP)
Texas A&M University
College of Veterinary Medicine
Dept. of Vet. Anatomy & Public Health
College Station, TX  77868-4458

>>> "Yandl, Emily" <> 08/18 10:08 AM >>>
Hi histonetters,

Does anyone out there have a good procedure for doing an H&E on a 50um
frozen section of a brain?  Right now, after fixation, we're starting in
water doing our normal routine.  Everything is quite muddy, however, but
because of the thickness of the section, I'm not sure it can be any clearer.
I'd just like to hear what other people are using for their staining times.

Thanks for any help!

Emily Yandl 

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