Re: H & E Staining Method

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From:"Sarah Christo" <>
To:<>, <Edna_J_Gonzalez/>
Date:Mon, 23 Aug 1999 08:03:33 -0500
Content-Type:text/plain; charset=US-ASCII

Dear Edna,
   If you need a lighter hematoxylin, why not use Mayer's?  If you insist on Harris, reduce the time.  

Sarah Christo, HT (ASCP)
Texas A&M University
College of Veterinary Medicine
Dept. of Vet. Anatomy & Public Health
College Station, TX  77868-4458

>>> <Edna_J_Gonzalez/> 08/19 12:57 PM >>>

I am working with pig skin and doing H&E regressive staining using Harris
Hematoxylin (non-acidified). The problem is that the hematoxylin is too
dark and I can't differentiate what I need, especially it is too dark in
the basal layer and the epidermis of the skin. I need a H&E Regressive
Method that will be lighter. Currently I am staining with hematoxylin for
10 minutes, which I think is too much, but this is what one of the methods
say. I know there are different H&E regressive methods (with different
times for Hematoxylin). Any suggestions?

Edna Gonzalez
PowderJect Vaccines

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