Re: H & E Staining Method

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From:"P. Emry" <emry@u.washington.edu>
To:Edna_J_Gonzalez/Powderject@powderject.com
Reply-To:
Date:Fri, 20 Aug 1999 17:23:15 -0700 (PDT)
Content-Type:TEXT/PLAIN; charset=US-ASCII

Hi Edna,

I do a lot of pig tissues and have been successful with Mayers hemo
for years.  It is like wine..gets better with age.  Of course there is a
limit.  I stain for 20 minutes max, but have gotten away with less with a
20 min wash with tap water.  Eosin over-stains easily so I dilute it and
give just a couple or 4 dips.

I used to prepare pig skin for burn treatments.  What are you using if
for?  The powder project?  Sounds interesting.

Hope Mayers works for you.

Trisha
Orthodontics Res. Lab
U of Washington, Seattle



On Thu, 19 Aug 1999 Edna_J_Gonzalez/Powderject@powderject.com wrote:

> 
> 
> I am working with pig skin and doing H&E regressive staining using Harris
> Hematoxylin (non-acidified). The problem is that the hematoxylin is too
> dark and I can't differentiate what I need, especially it is too dark in
> the basal layer and the epidermis of the skin. I need a H&E Regressive
> Method that will be lighter. Currently I am staining with hematoxylin for
> 10 minutes, which I think is too much, but this is what one of the methods
> say. I know there are different H&E regressive methods (with different
> times for Hematoxylin). Any suggestions?
> 
> Edna Gonzalez
> PowderJect Vaccines
> 
> 
> 
> 

Trisha  




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