Re: H & E Staining Method

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From:"Rae Staskiewicz" <raestask@galesburg.net>
To:<Edna_J_Gonzalez/Powderject@powderject.com>, "HistoNet Server" <histonet@pathology.swmed.edu>
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Date:Thu, 19 Aug 1999 18:57:17 -0500
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Edna,

    We do a lot of pig skin. Our H&E is a regressive stain using Harris
hematoxylin, however we use acidified. We stain for 61/2 minutes in Harris
followed by a 1 min wash in running water and 1 second in .5% acid alcohol.
If we can be of any assistance, please feel free to contact us at the
Galesburg Animal Disease Laboratory, Galesburg, IL 309-344-2451.

Rae Ann Staskiewicz
Galesburg Animal Disease Lab




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