Re: Golgi-Cox dark areas.

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From:Bruce A Rasmussen <brasmuss@osf1.gmu.edu>
To:Sarah Christo <schristo@cvm.tamu.edu>
Reply-To:
Date:Mon, 16 Aug 1999 10:46:34 -0400 (EDT)
Content-Type:TEXT/PLAIN; charset=US-ASCII


My protocol calls for 30 min in ammonium hydroxide.  Which should take
care  of the mercuric chloride?? Also I'm using sodium tungstate I wonder
if that is the bad guy.  Thanks for your input

Bruce


On Thu, 12 Aug 1999, Sarah Christo wrote:

> Dear Bruce,
>    I have been sectioning Golgi-Cox mouse brains in celloidin and we react them after sectioning with 5% ammonium hydroxide.  It sound like your silver is still reacting.  I'm no expert in this, I'm not doing the staining, just the sectioning and reacting, etc.  Just a thought.  
> 
> 
> Sarah Christo, HT (ASCP)
> Texas A&M University
> College of Veterinary Medicine
> Dept. of Vet. Anatomy & Public Health
> College Station, TX  77868-4458
> schristo@cvm.tamu.edu
> 
> >>> Bruce A Rasmussen <brasmuss@osf1.gmu.edu> 08/11 1:39 PM >>>
> 
> Greetings histo gurus,
> 
> Last spring I processed coronal rat brain sections with the Golgi-Cox
> method using the protocol of Kolb/Gibbs.  The tissue came out great BUT
> over time the sections are starting to turn black in the medial areas
> threatening our region of interest the hippocampus. We
> store the slides in the dark.  Anyone ever see this? Is there anyway to
> stabilize the slides?
> 
> Thanks in advance
> Bruce
> 
> 
> 
> 
> 
> --------------------------------------
> Bruce Rasmussen
> Predoc Fellow in Experimental Neuropsychology
> The Krasnow Institute for Advanced Study
> George Mason University
> Mail Stop 2A1
> Fairfax, VA 22030-4444
> Office:703-993-4358
> Lab:703-993-4369
> Home:703-765-4570
> Fax:703-993-4325
> ---------------------------------------
> 
> 
> 
> 
> 



--------------------------------------
Bruce Rasmussen
Predoc Fellow in Experimental Neuropsychology
The Krasnow Institute for Advanced Study
George Mason University
Mail Stop 2A1
Fairfax, VA 22030-4444
Office:703-993-4358
Lab:703-993-4369
Home:703-765-4570
Fax:703-993-4325
---------------------------------------




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