Re: Golgi-Cox dark areas.

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From:"Sarah Christo" <>
To:<>, <>
Date:Thu, 12 Aug 1999 08:50:53 -0500
Content-Type:text/plain; charset=US-ASCII

Dear Bruce,
   I have been sectioning Golgi-Cox mouse brains in celloidin and we react them after sectioning with 5% ammonium hydroxide.  It sound like your silver is still reacting.  I'm no expert in this, I'm not doing the staining, just the sectioning and reacting, etc.  Just a thought.  

Sarah Christo, HT (ASCP)
Texas A&M University
College of Veterinary Medicine
Dept. of Vet. Anatomy & Public Health
College Station, TX  77868-4458

>>> Bruce A Rasmussen <> 08/11 1:39 PM >>>

Greetings histo gurus,

Last spring I processed coronal rat brain sections with the Golgi-Cox
method using the protocol of Kolb/Gibbs.  The tissue came out great BUT
over time the sections are starting to turn black in the medial areas
threatening our region of interest the hippocampus. We
store the slides in the dark.  Anyone ever see this? Is there anyway to
stabilize the slides?

Thanks in advance

Bruce Rasmussen
Predoc Fellow in Experimental Neuropsychology
The Krasnow Institute for Advanced Study
George Mason University
Mail Stop 2A1
Fairfax, VA 22030-4444

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