Hi Andrea
We are currently fixing in 4% paraformaldehyde overnight then wash with pbs
and cryoprotect with 25% sucrose/PBS. We then freeze them and section. The
sections are allowed to air dry for 2 hours, then we wash in pbs and
coverslip with pbs and visualize using the FITC filter
Good Luck
Robbin


>I have read a few different ways of doing this so I ask: what protocols are
>people using to process GFP labeled tumors established in vivo? Just
>wondering ...


Robbin Newlin HT-ASCP
Manager Histopathology
The Burnham Institute
10901 North Torrey Pines rd.
La Jolla, Ca. 92037

Phone: (619) 455-6480 ext. 3552
E-Mail: rnewlin@ljcrf.edu