Re: GFP processing

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From:Robbin Newlin <>
Date:Fri, 20 Aug 1999 13:52:45 -0800
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Hi Andrea
We are currently fixing in 4% paraformaldehyde overnight then wash with pbs
and cryoprotect with 25% sucrose/PBS. We then freeze them and section. The
sections are allowed to air dry for 2 hours, then we wash in pbs and
coverslip with pbs and visualize using the FITC filter
Good Luck

>I have read a few different ways of doing this so I ask: what protocols are
>people using to process GFP labeled tumors established in vivo? Just
>wondering ...

Robbin Newlin HT-ASCP
Manager Histopathology
The Burnham Institute
10901 North Torrey Pines rd.
La Jolla, Ca. 92037

Phone: (619) 455-6480 ext. 3552

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