Re: Frozen sectioning fat tissue

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From:"Alan Bright" <>
To:"Anita Jennings" <>, <>
Date:Tue, 24 Aug 1999 15:13:44 +0100
Content-Type:text/plain; charset="iso-8859-1"

Dear Anita,

I addressed this point to Beth & HistoNet on the 18 August, we have been
fitting these controls to cryostats for over 20 years, they are fitted to
our clinical & research cryostats and they do not suffer from temperature
loss on the anti-roll plate, which would defeat the benefit of these

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margarets Way
PE18 6EB

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Web Site:

-----Original Message-----
From: Anita Jennings <>
To: <>
Date: Monday, August 23, 1999 10:35
Subject: RE: Frozen sectioning fat tissue

>I have seen many responses to your inquiry but have not seen where anyone
>uses a cryostat that has two temperature controls. My Zeiss has a chamber
>control and a block control. I can set the temps independently. Sometimes
>the guide plate (plastic) gets warm, but my magnetic blades never seem to
>need cooling down. When I do "fat" tissue I set the chamber to -25 and the
>chuck to -30. It works fine even with my hot little hands in the chamber
>since only the chamber feels my warmth, not the block. This may not be a
>solution to your problem but people who do a lot of fat tissue might want
>keep it in mind when the time comes to purchase a new cryostat. I am sure
>other vendors have what is sometimes referred to as a "research cryostat".

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