Re: Frozen secioning fat tissue
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| From: | "Tony Henwood" <henwood@mail.one.net.au> |
| To: | "HistoScientific" <histosci@shentel.net>, <histonet@pathology.swmed.edu>, "Alan Bright" <Bright@dial.pipex.com> |
| Reply-To: | |
| Date: | Wed, 25 Aug 1999 20:50:11 +0000 |
| Content-Type: | text/plain; charset=US-ASCII |
Dear Alan,
> I believe the problems you are having on sectioning fat are caused by one of
> two problems with your cryostat.
>
> Firstly, you need to be sectioning fat at around minus 30 deg.C. Rotary
> microtomes very often get very tight at these temperatures and stop
> advancing, if they are adjusted for the lower temperature ranges , then they
> do not perform so well for the majority of tissues that are sectioned in the
> minus 20 deg.C range.
Can you really cut frozen sections of fat? I'm impressed!!!!!
Regards, Tony
.
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital
Sydney, AUSTRALIA
http://www2.one.net.au/~henwood
http://www.pathsearch.com/homepages/TonyHenwood/default.html
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