Re: Frozen secioning fat tissue

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From:"Tony Henwood" <>
To:"HistoScientific" <>, <>, "Alan Bright" <>
Date:Wed, 25 Aug 1999 20:50:11 +0000
Content-Type:text/plain; charset=US-ASCII

Dear Alan,

> I believe the problems you are having on sectioning fat are caused by one of
> two problems with your cryostat.
> Firstly, you need to be sectioning fat at around minus 30 deg.C. Rotary
> microtomes very often get very tight at these temperatures and stop
> advancing, if they are adjusted for the lower temperature ranges , then they
> do not perform so well for the majority of tissues that are sectioned in the
> minus 20 deg.C range.

Can you really cut frozen sections of fat? I'm impressed!!!!!

Regards, Tony
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital

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