Re: Frozen secioning fat tissue

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From:"Alan Bright" <>
To:"Tony Henwood" <>, "HistoScientific" <>, <>
Date:Wed, 25 Aug 1999 16:23:36 +0100
Content-Type:text/plain; charset="iso-8859-1"

Dear Tony,

Yes, sectioning fat is not a problem with our cryostats, to back up my words
I have just sectioned a piece of pig fat 20 x 15mm including the skin @ 5
microns on our model OTF/AS/LT, chamber, microtome, knife, anti-roll plate &
specimen all at minus 38 degs.C. without problems, I even let one of our
electrical engineers who has never sectioned on a cryostat, cut a ribbon of
6 sections @ 5 microns, he had no problems and all the sections were good.

There are many of our cryostats in the Sydney area, if you would like me to
get some information on their location, I would be pleased to do so.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margarets Way
PE18 6EB

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Web Site:

-----Original Message-----
From: Tony Henwood <>
To: HistoScientific <>;
<>; Alan Bright <>
Date: Wednesday, August 25, 1999 11:51
Subject: Re: Frozen secioning fat tissue

Dear Alan,

> I believe the problems you are having on sectioning fat are caused by one
> two problems with your cryostat.
> Firstly, you need to be sectioning fat at around minus 30 deg.C. Rotary
> microtomes very often get very tight at these temperatures and stop
> advancing, if they are adjusted for the lower temperature ranges , then
> do not perform so well for the majority of tissues that are sectioned in
> minus 20 deg.C range.

Can you really cut frozen sections of fat? I'm impressed!!!!!

Regards, Tony
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital

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