Dear Dr.Montgomery,

I was very pleased to see your comments re. Vacuum Debris Collector & Fan
Cooler Hazards when fitted to cryostats.
These two points seemed to have been overlooked by many cryostat users in
the past, and I am pleased you raised this warning.

I was also pleased to see Peggy A. Wink make a most valid warning on the use
of cryogenic sprays, on infected tissue.

I await comments on these most important issues.

Alan Bright

Bright Instrument Co.Ltd.
St Margarets Way
Huntingdon
PE18 6EB
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: AlanBright@brightinstruments.com
Web Site: www.brightinstruments.com



-----Original Message-----
From: Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
Date: Thursday, August 19, 1999 10:04
Subject: Re: Frozen secioning fat tissue



> Perhaps spraying is not such a good idea in this instance with, as
>you say, Federal Registry and CAP prohibition. But, can I raise the matter
>of cryostats fitted with vacuum debris collector. Although they have
>filters do they really, or can they really, filter out prion particles. I
>would be a bit more afraid of a constant airflow out the rear of a cryostat
>than a quick blast of  coolant. Plus, some models of cryostat have fan
>coolers, again giving a gentle breeze of air around the cryostat. Open the
>lid and what will you get straight in the face and up the nose.
>Ian.
>
>

>Dr. Ian Montgomery,
>West Medical Building,
>University of Glasgow,
>Glasgow,
>G12 8QQ,
>Scotland.
>Tel: 0141 339 8855 Extn. 6602.
>Fax: 0141 330 4100.
>e-mail: ian.montgomery@bio.gla.ac.uk
>
>
>