Re: Frozen secioning fat tissue

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From:lpwenk@netquest.com (Wenk, Lee & Peggy)
To:Alan Bright <Bright@dial.pipex.com>
Reply-To:
Date:Thu, 19 Aug 1999 06:14:45 -0400
Content-Type:text/plain; charset=us-ascii



Alan Bright wrote:
> 
> Dear Beth,
> 
>  . . . To overcome the problem, you will have to spray the specimen, knife &
> anti-roll plate with a refrigerant spray (Cryospray) to get your fatty
> sections.

Oh NO!!!! NEVER use a cryogenic spray in a cryostat!!!

The Federal Registry and CAP prohibit this, as it can cause
droplet, dispersion, etc. of the tissue and air in the cryostat, 
which will then fly out of the cryostat, which you can then breathe.
Which can then infect you with whatever the patient(s) had in
the tissue fragments hanging around in your cryostat.

Lower the temperature of the cryostat, or freeze the tissue
in liquid nitrogen cooled to -50 degrees C (there are machines
available that do this. We bought ours from Shandon-Lipshaw.)
But do NOT use the cryogenic spray!

*********************

Peggy A. Wenk, HTL(ASCP)
William Beaumont Hospital
Royal Oak, MI 48073
> 
> Best Regards
> 
> Alan Bright
> 
> Bright Instrument Co.Ltd.
> St Margarets Way
> Huntingdon
> PE18 6EB
> England
> 
> Tel No:+44 (0)1480 454528
> Fax No:+44 (0)1480 456031
> Email: AlanBright@brightinstruments.com
> Web Site: www.brightinstruments.com
> 
> -----Original Message-----
> From: HistoScientific <histosci@shentel.net>
> To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
> Date: Tuesday, August 17, 1999 04:18
> Subject: Frozen secioning fat tissue
> 
> >Histonetters,
> >
> >We just completed a study consisting of mouse fat.  This fat was taken
> >from around such tissues as the uterus, heart, etc.  We used a cryostat
> >made by Microm and had it set at everywhere from -18 to -35 degrees.  We
> >left the tissue in the cryostat overnight and still no luck.  We could
> >not get a section no matter how thick or thin we tried cutting them.  We
> >then tried Histofreeze, still no luck.  To make a long story short, we
> >ended up giving "fat smears".  We have never had problems with our
> >cryostat and the techs that were working on this project had over a
> >century of experience with frozens.  Maybe Instrumedics could have come
> >to the rescue!
> >
> >Beth Poole
> >Histo-Scientific Research Labs.
> >107 Killmon Road
> >P.O. Box 30
> >Basye, VA  22810
> >(540)856-2222
> >histosci@shentel.net
> >
> >



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