Note
This method was also recommended in those days as a technique for the
demonstration of cells such as Langerhans cells in the skin. However, in our
hands this did not work well as the levels in these cells are low.
Barry

"Alton D. Floyd" wrote:

> Re: Falck-Hillarp method for catecholamines
>
> The procedures previously posted to the list are correct, according to
> the old literature, and the methods used in my laboratory in the late
> '60's.  Note that for the paraformaldehyde powder to produce gas, there
> must be some moisture present.  We always insured this by a very light
> misting of the powder surface, prior to placing the slides in the
> container, and sealing the container.  Remember not to open the container
> until you have it in a hood, since it will contain formaldehyde gas.
>
> The typical yellow-green fluorescence induced by this method is due to
> epinephrine and nor-epinephrine.  Dopamine containing cells give an
> orange fluorescence.  For a very nice compendium of SIF cells (Small,
> Intensely Flourescent cells), see the book by that name:  SIF Cells:
> Structure and Function of the Small, Intensely Fluorescent Sympathetic
> Cells, Ed., O. Eranko, 1976. Fogartry International Center Proceedings
> No. 30, DHEW Publication No. (NIH) 76-942.  This book was originally
> available from the U.S. Government Printing Office, Stock No.
> 017-053-00047-7.  I am sure it is out of print, but you may find it in a
> medical library.  It originally sold for $6.50 (ah, for the good old
> days)
> Alton D. Floyd
> 23126 South Shore Drive
> Edwardsburg, MI 49112
> (616) 699-7182 phone & fax