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From:"John C. Dennis" <>
Date:Fri, 27 Aug 1999 10:17:17 -0500 (CDT)
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You can make a 0.5 M stock solution, autoclave it, and, with care, keep it
for months.  I generally use 0.25 M EDTA of decalcification and dilute the
stock 1:1 with dH2O.

	146.12 g EDTA in approx. 900 ml dH2O
	while it's stirring, dump NaOH pellets, a few at a time, until the
	solution is clear.  Bring the solution up to 1 l with dH2O

	The resulting solution will be around pH 8-8.3.  

The sodium hydroxide is used to adjust the pH up because EDTA is not very
soluble in H2O.  The stock solution is not so basic that it will do
damage.  Take care in adding the NaOH pellets, though.  As the solution
begins to clarify add fewer and fewer pellets until the EDTA is just
dissolved.  This is a case where enough is just right and more is most
definitely not good.

Yours for solubility

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849

On Thu, 26 Aug 1999 wrote:

> Hello all:
>     I am looking for a formula to make EDTA.  If you have any suggestions I 
> would greatly appreciate your assistance.
> Jim Bradford
> Methodist Hospital
> Omaha, NE

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