Re: EDTA

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From:"Barry Rittman" <brittman@mail.db.uth.tmc.edu>
To:histonet@pathology.swmed.edu
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Date:Thu, 26 Aug 1999 19:21:41 -0700
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Jim,
		would you let us know what you wish to use it for? If for
demineralization need 5-10% disodium EDTA. preferably at pH 7.2-7.4. Do not
try to use EDTA itself as it is fairly insoluble in water, dissolve the
disodium salt and adjust with dilute sodium hydroxide to the correct pH.
The solution you end up with is really a mixture of di- and tri-sodium EDTA.
Barry

At 04:03 PM 08/26/1999 EDT, you wrote:
>Hello all:
>
>    I am looking for a formula to make EDTA.  If you have any suggestions I 
>would greatly appreciate your assistance.
>
>Jim Bradford
>Methodist Hospital
>Omaha, NE
>
>



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