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From:C & J Haley <>, HistoNet Server <>
Date:Thu, 26 Aug 1999 18:01:21 -0500
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Hi Jim:

We purchase EDTA from Sigma (catalog#E-5134).  We use a 1mM (0.37g/1000ml
distilled water)  solution at pH 8.0 for our microwave pretreatment of certain
antibodies. The pH of this solution will fluctuate, so always check it before
using it.
Hope this is what you needed.

Jane Haley
Barnes-Jewish Hospital
St. Louis, Mo. wrote:

> Hello all:
>     I am looking for a formula to make EDTA.  If you have any suggestions I
> would greatly appreciate your assistance.
> Jim Bradford
> Methodist Hospital
> Omaha, NE

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