Dear Phyllis,

We use pen-fix as a post-fixative after initial fixation in 10% NBF, and
have had no problems with ER, PR or Her-2.


Diana Goodwin,  HT
Trenton,  NJ


> ----------
> From: 	PHarris379@aol.com[SMTP:PHarris379@aol.com]
> Sent: 	Monday, August 23, 1999 9:53 PM
> To: 	histonet@pathology.swmed.edu
> Subject: 	alcoholic formalin
> 
> Does anyone in Histoland use alcoholic formalin (pen fix) as a primary
> 
> fixative, that does er/pr and her2? Most of our antibodies do well
> with the 
> penfix, but I am curious to hear of any tips using this fixative with
> nuclear 
> antigens.
>   Thanks,
> Phyllis
>