RE: LR White/MMA thin sections

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From:"Sarah Christo" <>
To:<>, <>, <>
Date:Mon, 23 Aug 1999 15:26:40 -0500
Content-Type:text/plain; charset=US-ASCII

Dear Jennifer,
   I tried it this week and it works fine.   I like the peel-a-way better than smashing glass.  The JB-4 molds also work.  Thanks for the reply.  Sarah

Sarah Christo, HT (ASCP)
Texas A&M University
College of Veterinary Medicine
Dept. of Vet. Anatomy & Public Health
College Station, TX  77868-4458

>>> "MacDonald, Jennifer" <> 08/20 1:53 PM >>>
Years ago we use the peel-a-way mold for MMA embedding.  I would assume that
nothing has changed??  

(909) 985-2811 ext. 4148 

> ----------
> From: 	Linda Jenkins[] 
> Sent: 	Friday, August 20, 1999 10:32 AM
> To: 
> Subject: 	LR White/MMA thin sections
> Dear Sarah,
> 	In respnse to your question:
> 	 "Are the peel-a-way molds also used for MMA when doing thin
> sections?  How are they clamped into the microtome?  I have a Sorval
> microtome I use for the plastics.  I assume glass knives are used for thin
> sectioning MMA, is that correct?"
> 	Good question!  I haven't tried the Peel Away Molds for MMA.
> However, I bet someone out in histoland has - maybe they will share.
> Suggest trying it and put mold in an aluminum foil, make-shift pan in case
> it dissolves or distorts.  You see, I do very large embedments in MMA and
> use tupperware (yes, let's do have a party), rubbermaid, disposable 9 x 13
> cake pans, whatever.  If the peel away molds work, I don't see why you
> couldn't use the embedding rings in the same manner as the GMA method.
> Or,
> you can attach an embedding ring to the back of the block with super-glue.
> Yes, you can use glass knives, but I use the tungsten-carbide blades due
> to
> the large size of my specimens.  I also section these MMA blocks on a
> Polycut E automated microtome.  I don't think you can section MMA blocks
> on
> a manual microtome - automated being the KEY word here.
> 	Armadillo ears???  What will we find to cut next:-)
> 	Linda
> *********************************
> Linda Jenkins, HT
> Clemson University
> Department of Bioengineering
> Clemson, SC
> **********************************

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