RE: LR White/MMA thin sections
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From: | "MacDonald, Jennifer" <jmacdonald@sach.org> |
To: | histonet@pathology.swmed.edu, "'Linda Jenkins'" <jlinda@ces.clemson.edu> |
Reply-To: | |
Date: | Fri, 20 Aug 1999 11:53:57 -0700 |
Content-Type: | text/plain |
Years ago we use the peel-a-way mold for MMA embedding. I would assume that
nothing has changed??
Jennifer
(909) 985-2811 ext. 4148
jmacdonald@sach.org
> ----------
> From: Linda Jenkins[SMTP:jlinda@ces.clemson.edu]
> Sent: Friday, August 20, 1999 10:32 AM
> To: histonet@pathology.swmed.edu
> Subject: LR White/MMA thin sections
>
> Dear Sarah,
> In respnse to your question:
>
> "Are the peel-a-way molds also used for MMA when doing thin
> sections? How are they clamped into the microtome? I have a Sorval
> microtome I use for the plastics. I assume glass knives are used for thin
> sectioning MMA, is that correct?"
>
> Good question! I haven't tried the Peel Away Molds for MMA.
> However, I bet someone out in histoland has - maybe they will share.
> Suggest trying it and put mold in an aluminum foil, make-shift pan in case
> it dissolves or distorts. You see, I do very large embedments in MMA and
> use tupperware (yes, let's do have a party), rubbermaid, disposable 9 x 13
> cake pans, whatever. If the peel away molds work, I don't see why you
> couldn't use the embedding rings in the same manner as the GMA method.
> Or,
> you can attach an embedding ring to the back of the block with super-glue.
> Yes, you can use glass knives, but I use the tungsten-carbide blades due
> to
> the large size of my specimens. I also section these MMA blocks on a
> Polycut E automated microtome. I don't think you can section MMA blocks
> on
> a manual microtome - automated being the KEY word here.
> Armadillo ears??? What will we find to cut next:-)
> Linda
> *********************************
> Linda Jenkins, HT
> Clemson University
> Department of Bioengineering
> Clemson, SC
> **********************************
>
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